Seladelpar

The Selective Peroxisome Proliferator– Activated Receptor-Delta Agonist Seladelpar Reverses Nonalcoholic Steatohepatitis Pathology by Abrogating Lipotoxicity in Diabetic Obese Mice

Lipotoxicity associated with insulin resistance is central to nonalcoholic steatohepatitis (NASH) pathogenesis. To date, only weight loss fully reverses NASH pathology, but mixed peroxisome proliferator–activated receptor-alpha/delta (PPAR-a/d) ago- nists show some efficacy. Seladelpar (MBX-8025), a selective PPAR-d agonist, improves atherogenic dyslipidemia. We there- fore used this agent to test whether selective PPAR-d activation can reverse hepatic lipotoxicity and NASH in an obese, dyslipidemic, and diabetic mouse model. From weaning, female Alms1 mutant (foz/foz) mice and wild-type littermates were fed an atherogenic diet for 16 weeks; groups (n 5 8-12) were then randomized to receive MBX-8025 (10 mg/kg) or vehicle (1% methylcellulose) by gavage for 8 weeks. Despite minimally altering body weight, MBX-8025 normalized hyperglycemia, hyper- insulinemia, and glucose disposal in foz/foz mice. Serum alanine aminotransferase ranged 300-600 U/L in vehicle-treated foz/ foz mice; MBX-8025 reduced alanine aminotransferase by 50%. In addition, MBX-8025 normalized serum lipids and hepatic levels of free cholesterol and other lipotoxic lipids that were increased in vehicle-treated foz/foz versus wild-type mice. This abol- ished hepatocyte ballooning and apoptosis, substantially reduced steatosis and liver inflammation, and improved liver fibrosis. In vehicle-treated foz/foz mice, the mean nonalcoholic fatty liver disease activity score was 6.9, indicating NASH; MBX-8025 reversed NASH in all foz/foz mice (nonalcoholic fatty liver disease activity score 3.13). Conclusion: Seladelpar improves insulin sensitivity and reverses dyslipidemia and hepatic storage of lipotoxic lipids to improve NASH pathology in atherogenic diet–fed obese diabetic mice. Selective PPAR-d agonists act independently of weight reduction, but counter lipotoxicity related to insulin resistance, thereby providing a novel therapy for NASH. (Hepatology Communications 2017; 00:000–000) onalcoholic fatty liver disease (NAFLD) results from overnutrition, particularly in those with a family history of or established type 2 diabetes (T2D) and in people with metabolic syndrome and cardiovascular risk.(1,2) Thus, in genetically predisposed individuals, constant energy surplus and bodily insulin resistance increase hepatic lipid partitioning to result in steatosis (total lipids 5% of liver weight).(1,2) When hepatic lipid is comprised principally of triglyceride, liver pathology is confined to simple steatosis; but the presence of other specific lipid fractions (free fatty acids [FAs], diacylglycerides, free cholesterol [FC]) can injure hepatocytes in a process termed lipotoxicity.(3-6) Pathological responses to hepatic lipotoxicity include a mixed cellular inflamma- tory infiltrate focused on injured hepatocytes, hepato- cyte ballooning and cell death, and liver fibrosis. These are the hallmarks of steatohepatitis, referred to as non- alcoholic steatohepatitis (NASH) when alcohol intake is <70 g/week in women and <140 g/week in men.(2) Ten percent to 25% of NAFLD patients develop NASH. It is more likely among those with a more severe metabolic phenotype (established T2D, meta- bolic syndrome) and/or a family history of T2D or NAFLD/cirrhosis.(1,7) Childhood obesity, typically genetic and environmental in origin, also predicts later onset of NASH.(2,4,8) The distinction between NASH and simple steatosis is clinically important because NASH is often associated with fibrosis, the component of NAFLD pathology predictive of progression to cir- rhosis.(9) Although NASH is a highly prevalent, serious disease, there is no currently approved drug ther- apy.(1,2,6,7) While reversing consequences of metabolic disorder has been attempted to target NASH, the only highly effective strategies have been bariatric surgery and lifestyle changes; reducing body weight 10% reverses NASH pathology, including liver fibrosis, but is difficult to achieve.(10-15) Pharmacologically, pathways targeted to improve NASH pathology are aimed at “movers” of metabolic obesity: glucagon-like peptide 1 receptor agonists, adiponectin receptor agonists, gluco- kinase activity modulators, and selective peroxisome proliferator–activated receptor (PPAR) agonists.(14-17) PPARs are a nuclear receptor superfamily of ligand- inducible transcription factors(18) that induce gene expression involved in energy homeostasis, as well as inflammation pathways. PPAR-a plays a pivotal role in hepatic lipid and lipoprotein metabolism, particularly in male rodents. Targeting this receptor is used to treat mixed dyslipidemia and hypertriglyceridemia(16,18) but is not effective against NASH. PPAR-c is the master reg- ulator of adipogenesis and lipogenic pathways in adipo- cytes; the thiazolidinedione PPAR-c agonists improve peripheral and hepatic insulin sensitivity(16,18,19) and have some efficacy in NASH(14) but without reversal of NASH pathology. PPAR-d is expressed by multiple tis- sues including liver, skeletal muscle, intestine, and mac- rophages.(20) PPAR-d agonists, such as GW501516, may exert beneficial effects on hyperglycemia, hyperinsu- linemia, and NAFLD.(20,21) Thus, GW501516 decreased serum triglycerides, increased high-density lipoprotein, and reduced hepatic fat.(22,23) Recent atten- tion has turned to GFT-505 (elafibranor), a dual- activator of PPAR-a/d with a bias toward PPAR-a (50% effective concentration 5 45 nM versus 175 nM for PPAR-d).(15,24) The relative risk and benefits of tar- geting PPAR-a versus PPAR-d have not been eluci- dated in long-term studies, and GFT-505 failed to activate PPAR-d in peripheral tissues in obese insulin- resistant patients.(24) Seladelpar (also known as MBX-8025) is a novel, potent, and selective PPAR-d agonist (50% effect con- centration human PPAR-d 5 2 nM, PPAR-a 5 1,600 nM) that demonstrates favorable effects on insu- lin resistance, diabetes, and atherogenic dyslipide- mia.(23,25-27) In an 8-week placebo-controlled, active comparator study of obese patients with mixed dyslipi- demia, MBX-8025 reduced low-density lipoprotein cholesterol and triglyceride, accompanied by reduced concentrations of small, dense low-density lipoprotein particles.(25,26) In the present study, we addressed the effects of MBX-8025 treatment on insulin resistance, glucose tolerance, hepatic lipid fractions, and liver pathology in wild-type (Wt) and appetite-defective (Alms1 mutant foz/foz) mice fed an atherogenic diet. foz/foz mice develop obesity complicated by T2D and resemble humans with NASH by exhibiting high blood pressure, atherogenic dyslipidemia, hypoadiponectinemia, and steatohepatitis complicated by pericellular fibrosis.(3,28-30) Experimental procedures were approved by the Aus- tralian National University Animal Ethics Committee (pA2012/20). From weaning (week 4), Alms1 mutant (foz/foz) NOD.B10 mice or Wt littermates (female mice in both groups) were fed an atherogenic diet (23% fat, 0.2% cholesterol and 45% simple carbohy- drate; 4.78 kcal/g digestible energy; SpecialtyFeeds, Glen Forrest, Australia) ad libitum for 16 weeks, after which groups were randomized (n 5 8-12 mice/group) to once-a-day oral administration (by gavage) for 8 weeks of MBX-8025 (10 mg/kg in 1% methylcellu- lose) or vehicle (controls). Animals were housed under 12-hour light/dark cycle and constant temperature of 228C and received maximal humane care. Seladelpar (MBX-8025; Supporting Fig. S1) was synthesized and characterized as described.(31) We measured body weight each week to calculate amounts of MBX-8025 or vehicle for gavage. One week before sacrifice (week 27), we performed an intraperitoneal glucose tolerance test after a 4-hour fast; following glu- cose injection (2 g/kg lean body mass), blood glucose was measured at 0, 15, 30, 60, and 120 minutes by glucometer (Accu-Chek Advantage; Roche Diagnos- tics, Mannheim, Germany). One week later, mice were fasted for 4 hours and anesthetized (100 mg/kg ketamine, 16 mg/kg xylazine), and blood and liver were harvested. Circulating blood insulin was assessed using an enzyme-linked immunosorbent assay kit (EMD Millipore, Darmstadt, Germany). The homeo- static model assessment of insulin resistance was calcu- lated as [fasting insulin (ng/mL) 3 25 3 fasting glucose (mmol/L)]/22.5. Serum alanine aminotrans- ferase (ALT), total triglyceride, and cholesterol were analyzed by ACT Pathology (The Canberra Hospital) using multichannel autoanalyzer.ASSESSMENT OF HEPATIC LIPIDS AND LIVER HISTOLOGYTotal neutral lipid (NL) content was assessed by staining of homogenized frozen livers (50 mg)with oil red O (3.67 mM), then measuring the absorbance (at 450 nm) of eluted dye. For lipido- mic analyses, liver total lipids were extracted by a modified Folch extraction protocol. Total FAs were analyzed as methyl esters by gas chromatog- raphy on a 60-m HP-INNOWax column pro- grammed from 808C to 2208C (Agilent Technologies, Santa Clara, CA) in a GC-17A(Shimadzu Scientific, Kyoto, Japan). The NL frac- tion was obtained from the total lipid extract by solid-phase separation on an Agilent Bond Elut Si cartridge (500 mg), eluting with 1% acetic acid in chloroform. The eluate was evaporated to dryness and the residue redissolved in 1% acetic acid in hexane for high-pressure liquid chromatography on a LiChrospher DIOL column (Alltech, Deerfield, IL) with detection by an LT-II light-scattering detector (Shimadzu). Hepatic macrosteatosis, nec- roinflammation, ballooning scores, and their sum (overall NAFLD activity score) were determined by an expert (blinded) liver pathologist on hema- toxylin and eosin–stained liver sections, according to the system devised for human NAFLD.(30) To quantify liver fibrosis, sirius red–stained sections were analyzed by collagen densitometry.(3,29) Hepatocyte apoptosis and proliferation were visual- ized by immunostaining of caspase-cleaved cyto- keratin-18 fragment (M30) and proliferating cell nuclear antigen (PCNA), respectively. Epidermal growth factor–like module–containing mucin-like hormone receptor-like 1 (F4/80) was used to detect hepatic macrophage crown-like structures (CLSs).(28)QUANTIFICATION OF HEPATIC GENE EXPRESSIONLiver homogenates were prepared in TRI reagent, total RNA was isolated,(29) and complementary DNA was obtained from 1 lg total RNA.(13) mRNA levels were quantified by real-time polymerase chain- reaction using SYBR green primers (Sigma-Aldrich, St. Louis, MO), normalizing data to the geometric mean of b-actin and Gapdh. Liver proteins were ana- lyzed as described.(13)Parametric data are presented as mean 6 SEM. Most experimental procedures were performed induplicate, with a minimum of eight samples/group. Data significance was assessed by one-way or two- way analysis of variance, followed by Bonferroni’s post hoc analysis. Group differences were considered significant when P < 0.05. Other than for assessment of liver pathology, the investigators involved in this research were not blinded during the experimental procedures. Results In atherogenic diet-fed Wt mice, administration of MBX-8025 reduced body weight by ~18% (P < 0.05; Fig. 1A). In contrast, MBX-8025 produced minimal effect on body weight in atherogenic diet–fed foz/foz mice (Fig. 1A). As expected,(3,28) these animals developedsevere hyperglycemia, hyperinsulinemia, and whole-body insulin resistance after 16 weeks (P < 0.05; Fig. 1B); MBX-8025 strikingly improved these indices (P < 0.05).After intraperitoneal glucose injection, blood glucose reached ~32 mmol/L in vehicle-treated versus ~14 mmol/L in MBX-8025-treated foz/foz mice (P < 0.05; Fig. 1C); the area under the blood glucose disappearancecurve was correspondingly lower in MBX-8025-treated foz/foz mice (P < 0.05; Fig. 1C). MBX-8025 produced a proportionally similar effect on glucose handling in ath- erogenic diet–fed Wt mice (P < 0.05; Fig. 1C).After 16 weeks of atherogenic dietary intake, serumALT was higher in foz/foz than Wt mice (P < 0.05; Fig. 1D). MBX-8025 lowered serum ALT levels in foz/foz mice (P < 0.05; Fig. 1D) and similarly (but not significantly) in Wt mice (Fig. 1D). Serum cholesterolwas markedly increased in foz/foz compared to Wt mice (P < 0.05; Fig. 1E); similar to the effects seen in patients with hypercholesterolemia,(25,26) MBX-8025normalized serum cholesterol. Serum triglyceride con- centrations were similar between foz/foz and Wt mice at 20 weeks of age, and again similar to patients with elevated triglycerides.(25,26) MBX-8025 decreased tri- glycerides in both genotypes (P < 0.05; Fig. 1F).MBX-8025 NORMALIZED HEPATIC LIPID PARTITIONING AND REVERSED NASH PATHOLOGY IN ATHEROGENIC DIET–FED foz/foz MICEHepatic total NLs (oil red O incorporation) were higher in atherogenic diet–fed foz/foz versus Wt mice (P < 0.05); MBX-8025 profoundly reducedtotal NLs in foz/foz as well as Wt mice (P < 0.05;Fig. 2A). This was reflected by chromatographicanalyses of hepatic triglyceride, diacylglycerol, and total FA content (P < 0.05; Fig. 2B-D). Hepatic FC increases in human NASH,(5) and as reported,(3,32) hepatic FC and cholesteryl esters were strikingly increased in atherogenic diet–fed foz/foz mice com- pared to Wt (P < 0.05; Fig. 2E); MBX-8025 reduced both FC and cholesteryl ester content. In addition, we measured total amounts of individual hepatic FA levels such as palmitic, palmitoleic, oleic,stearic, linoleic, and docosahexaenoic acid levels; MBX-8025 consistently reduced hepatic levels of these molecules in foz/foz mice (P < 0.05; Support- ing Fig. S2). As indicated by hematoxylin and eosin staining, macrovesicular steatosis was mild in Wt andsevere in foz/foz mouse livers (Fig. 2F and Table 1). MBX-8025 appeared to reduce macrovesicular stea- tosis in both lines (Fig. 2F), nearly halving the stea- tosis score in foz/foz mouse livers (Table 1). MBX-8025 abolished hepatocyte ballooning (P < 0.05; Fig. 2F and Table 1). The overall NAFLD activity score was ~6.9 in vehicle-treated foz/foz mice, indi-cating “definite NASH” in all foz/foz mice (Table 1). MBX-8025 decreased the NAFLD activity score by ~50% and reversed NASH in 8/8 mice. Thus, all PPAR-d agonist–treated, atherogenic diet–fed foz/foz mice showed simple steatosis (Fig. 2F and Table 1).MBX-8025 REDUCED HEPATOCELLULAR APOPTOSIS AND LIVER INFLAMMATION IN ATHEROGENIC DIET–FED foz/foz MICEfoz/foz mice developed hepatomegaly; consistent with the known effects of PPAR-d agonists on liver, MBX-8025 did not affect this increase (Table 1). M30–positive hepatocytes, reflecting apoptosis,(33) were more abundant in foz/foz mouse livers than Wt(P < 0.05; Fig. 3A); MBX-8025 normalized apoptosis levels to those of Wt controls (P < 0.05). Liver injuryprovokes an adaptive hepatocellular proliferative response. Thus, the number of PCNA–positive hepa- tocyte nuclei was higher in foz/foz mouse livers com- pared to Wt; MBX-8025 suppressed this to Wt levels(P < 0.05; Fig. 3B). While NASH pathology is of clinical significance in NAFLD, the histological factorthat correlates best with progression to cirrhosis is liver fibrosis.(9) As determined by the sirius red–positive (collagen-stained) area, liver fibrosis was barely evident after 24 weeks of atherogenic dietary intake in Wt mice (Fig. 3C), but by this time foz/foz mice exhibited sub- stantial fibrosis (Fig. 3C), as noted(28,29); MBX-8025 significantly reduced sirius red–positive areas in foz/fozmice (P < 0.05; Fig. 3C). This change was supported by a trend (not significant) for a decrease in hepatic alpha-smooth muscle actin mRNA levels in MBX-8025-treated foz/foz mice and reductions in Col1-a1, Ctgf, and Timp1 transcripts (P < 0.05; Supporting Fig. S3).The number of contiguous cells positive for F4/80 and forming CLSs indicates a focal proinflammatory response to injured hepatocytes. There were no CLSs in Wt mouse livers (P < 0.05; Fig. 3D), but the num- ber was very high in livers of vehicle-treated foz/fozmice with NASH. MBX-8025 diminished the hepatic CLS number in foz/foz mouse livers compared to vehicle-treated controls (P < 0.05; Fig. 3D). Despite MBX-8025 treatment resulting in a substantial reduc- tion in steatosis and ballooning scores and an impres-sive decrease in CLSs around injured hepatocytes, there was only a modest decline in the inflammation score (Table 1). We considered it possible that macro- phages accumulating during tissue repair could con- found interpretation of the inflammatory score in this context. We therefore sought other evidence for anti- inflammatory effects of MBX-8025 by measuring gene expression for chemokines and chemokine receptors pertinent to hepatic inflammation. Mcp1 mRNA washigher in foz/foz than Wt mouse liver (P < 0.05; Sup- porting Fig. S4A); MBX-8025 suppressed hepatic expression of this gene but did not affect expression ofits receptor Ccr2 (Supporting Fig. S4B). Transcripts for Ccl5 and Cx3Cr1 were also higher in livers of foz/ foz versus Wt mice (P < 0.05); MBX-8025 reduced Cx3Cr1 mRNA in foz/foz and Ccl5 mRNA in Wt mouse livers (P < 0.05; Supporting Fig. S4C,D).Cd11b and Cd11c mRNA levels were also increased infoz/foz mouse livers (versus Wt) (Supporting Fig. S4E, F); MBX-8025 lowered Cd11c, but not Cd11b, mRNA.TRANSCRIPT EXPRESSION OF GENES INVOLVED IN HEPATIC LIPID METABOLISM WAS INCREASED BY MBX-8025 IN ATHEROGENIC DIET–FED foz/foz MICEWe considered it likely that lower hepatic content of lipotoxic and proinflammatory lipid species such as FC, diacylglycerol, and FAs could influence NAFLD pathology in atherogenic diet–fed foz/foz mice. We therefore studied genes whose expression is regulatedby PPAR-a, PPAR-d, or both, in some cases at the protein as well as mRNA level. Pnpla2 promotes mito- chondrial function, so as to increase FA oxidation in the cell.(34) In the present study, MBX-8025 increasedhepatic expression of Pnpla2 gene (mRNA and pro- tein) in both genotypes (P < 0.05; Fig. 4A). The genes Cidea and Plin2 regulate lipid droplet formation andlipid storage(35); MBX-8025 treatment increased hepatic Cidea and Plin2 mRNA levels in both geno- types (P < 0.05; Fig. 4B). mRNA levels of the FA oxi-dation factors Acadl and Cpt2 were also increased (P <0.05) in livers of MBX-8025-treated versus vehicle-treated foz/foz mice (Fig. 4C; no treatment effect on Cpt1 mRNA [data not shown]). Peroxisomal Acox1 and microsomal Cyp4a10 mRNA expression was induced by MBX-8025 in both genotypes (P < 0.05;Fig. 4C), with no differences between vehicle-treatedfoz/foz and Wt mice (Fig. 4C). In addition, hepatic mRNA levels of Ucp2 and Pdk4, which are involved in mitochondrial metabolism, were substantially increased by MBX-8025 in both genotypes (P < 0.05; Fig. 4D).Hepatic mRNA expression of Abca1 was increased inMBX-8025-treated Wt mice but not influenced by MBX-8025 in foz/foz mice (Fig. 4E), and any change in Angptl4 in both lines did not reach statistical signifi- cance (Fig. 4E). Discussion In individuals with genetic predisposition, especially those with a family history of T2D,(1,7) excess calorie intake is complicated by insulin resistance. The resul- tant hyperinsulinemia up-regulates hepatic FA uptake and synthesis in the liver.(36) In addition, hyperinsuli- nemia programs the hepatic uptake of FC and down- regulates its biotransformation into bile acids.(3,5) Such dysregulation of hepatic cholesterol turnover was first documented in the appetite-dysregulated Alms1 mutant (foz/foz) mice used here,(3,28) and as later con- firmed in human NASH,(5) it results in accumulation of FC. FC and possibly other lipid and bile acid– related molecules have been shown to cause lipotoxicity in primary hepatocytes.(32,37) Physical activity and weight loss improve insulin sensitivity and so correct the hepatic accumulation of lipotoxic lipids.(3,10,29) However, attempts to combat hepatic lipid partitioning and promote insulin sensitization pharmacologically have been only partially effective, in the case of PPAR- c and farnesoid X receptor agonists,(11-13) or ineffective (metformin and PPAR-a agonists).(11)The most important finding of the present study is that a selective PPAR-d agonist, seladelpar (MBX- 8025), exerts powerful effects on hepatic lipid parti- tioning in the face of morbid obesity, complicated by diabetes and metabolic syndrome in mice. MBX-8025 returns hepatic levels of candidate lipotoxic lipids (FC, diacylglycerol, and FAs) to normal, and this alteration in liver lipid profile was achieved with minimal or no reduction in body weight. Further, restitution of physi- ological hepatic lipid levels was associated with improvement of steatosis, resolution of hepatocyte bal- looning and apoptosis, and reduction in macrophage recruitment and Kupffer cell activation to form CLSs. This culminated in the reversal of NASH pathology, with significant improvement in liver fibrosis.A key question is how does a selective PPAR-d ago- nist act independently of weight loss to reduce circulating and liver tissue cholesterol and triglyceride levels in obese diabetic mice? We attribute these changes to an MBX-8025-regulated increase in insulin sensitivity and enhanced energy use, changes that have been demonstrated by PPAR-d stimulation with tool agents that have not progressed to clinical stud- ies.(38,39) Thus, MBX-8025 lowered fasting blood glu- cose, decreased serum insulin concentrations and whole-body insulin resistance (homeostatic model assessment of insulin resistance), and normalized glu- cose disposition after an intraperitoneal load. MBX- 8025 has been shown to improve atherogenic dyslipi- demia, particularly lowering serum low-density lipo- protein cholesterol in humans and rodents(23,25-27); the present study extends this by demonstrating that enhanced insulin sensitivity is part of the mechanism. Others have shown that mixed PPAR-b/d agonistsincrease energy use by skeletal muscle(38,40); for exam- ple, GW501516, a prototypic PPAR-d agonist, reduces glucose use by muscle cells while increasing mitochondrial uncoupling and lipid use.(41) Clinical trials of this agent were terminated because of safety concerns.(42,43) By contrast, MBX-8025, a potent PPAR-d agonist with high binding affinity to its receptor, caused no side effects during this study, nor have they been a feature of clinical trials to date.(25-27)In the present study, MBX-8025 reduced the amount of hepatic NLs in atherogenic diet–fed Wt mice, which is a simple dietary model of steatosis. The atherogenic diet–fed foz/foz mouse is a multifactorial model of NASH in which both a dietary component and genetically determined drive to overeat exert inter- active effects. MBX-8025 reduced liver total NLs in this more severe model compared to levels found in Wt mice. To understand whether this reduction was attributable to a direct effect of MBX-8025 on the liver, we assayed PPAR-d target molecules concerned with intracellular lipid metabolism and lipid droplet formation and function. The increase in PNPLA2 protein expression produced by MBX-8025 is consis- tent with the known role of PPAR-d in FA oxidation and provides a rational explanation for the reduction of cellular triglyceride levels and decreased steatosis; PNPLA2 protein regulates lipolysis so as to lower tri- glyceride levels and provide substrate for FA oxida- tion.(34) In addition, MBX-8025 increased mRNA expression of lipid droplet factors such as Plin2 and Cidea. Cidea expression correlates with “browning” of adipocytes and fatty hepatocytes, a phenotypic switch in which cells become metabolically more active.(35) MBX-8025 also increased expression of FA oxidation genes Acadl, Cpt2 (mitochondrial), Acox1 (peroxi- somal), and Cyp4a10 (microsomal) in foz/foz mouse livers. We interpret the profile of these changes as indicating that MBX-8025 treatment could trigger hepatocytes to become metabolically more active. This proposal was supported by increased transcripts of the mitochondrial factors Ucp2 and Pdk4. In light of these data, it seems possible that MBX-8025 acts on hepato- cytes to reduce steatosis by activating pathways for enhancing lipid droplet storage, possibly also to pro- mote cellular lipid metabolism and oxidation.The predominant inflammatory cell types in livers with NASH are F4/80–positive Kupffer cells and infil- trated macrophages. Such activated macrophages coa- lesce around injured hepatocytes in a crown-like structure pattern. MBX-8025 treatment substantially lowered the number of CLSs in liver sections of foz/fozmice. It is salient to note that PPAR-d is expressed in macrophages, where it promotes their polarization toward an anti-inflammatory phenotype.(21) As expected,(3,28,29) the NASH-related liver inflammation in foz/foz mice fed an atherogenic diet was associated with increased expression of macrophage factors such as Mcp1, Cx3Cr1, and Cd11c mRNA; and MBX-8025 decreased their expression in foz/foz mouse livers. Inflammation-driven fibrosis is an important aspect of NASH clinically,(3,27,28) and MBX-8025 showed promising effects on reducing fibrosis, as shown by collagen densitometry. However, further study is required to establish whether MBX-8025 arrests fibro- sis deposition by extinguishing NASH pathology or whether it has effects on matrix-depositing cells, growth factors, and macrophages that can promote liver fibrosis resolution. In summary, we provide novel evidence that the selective PPAR-d agonist MBX-8025 reduces hepatic lipid partitioning, near totally correcting levels of lip- otoxic lipids in mice with metabolic obesity associated with insulin resistance and diabetes. The beneficial effects of MBX-8025 result from effects on extrahe- patic metabolism that contribute to improved insulin sensitivity and glucose handling and possibly from direct effects on hepatic lipid storage and oxidation. By showing a close correlation between removal of FC and/or other lipotoxic lipids and resolution of hepatocyte injury and NASH pathology, the present data reinforce a mechanistic view that NASH is a form of liver lip toxicity.(3-5) The translational poten- tial of the present findings is considerable as today there is still no drug licensed for use in NASH and none in clinical trial that reverses NASH in the majority of cases. Should the reversal of NASH docu- mented in this murine model, which replicates the predisposing metabolic milieu and liver pathology of human NASH, be reproduced in patients, seladelpar (MBX-8025) and other selective PPAR-d agonists could provide a mechanism-based, novel approach to treat NASH.